5CA7
Human DNA polymerase lambda- MgdGTP binary and complex with 6 paired DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-12-07 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 84.113, 84.113, 399.442 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.311 - 2.522 |
| R-factor | 0.211 |
| Rwork | 0.208 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xsl |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.610 |
| High resolution limit [Å] | 2.520 | 5.420 | 2.520 |
| Rmerge | 0.093 | 0.042 | 0.694 |
| Rmeas | 0.100 | 0.047 | 0.752 |
| Rpim | 0.039 | 0.019 | 0.288 |
| Total number of observations | 195094 | ||
| Number of reflections | 29246 | ||
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 98.3 | 97.6 | 94.6 |
| Redundancy | 6.7 | 5.9 | 6.6 |
| CC(1/2) | 0.998 | 0.849 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M Lithium Sulfate monohydrate, 0.1M HEPES-Sodium, 0.1M Potassium Sodium Tartrate |
