5CA7

Human DNA polymerase lambda- MgdGTP binary and complex with 6 paired DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	95
Detector technology	CCD
Collection date	2013-12-07
Detector	RAYONIX MX300HE
Wavelength(s)	0.9000
Spacegroup name	P 65 2 2
Unit cell lengths	84.113, 84.113, 399.442
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.311 - 2.522
R-factor	0.211
Rwork	0.208
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xsl
RMSD bond length	0.002
RMSD bond angle	0.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.610
High resolution limit [Å]	2.520	5.420	2.520
Rmerge	0.093	0.042	0.694
Rmeas	0.100	0.047	0.752
Rpim	0.039	0.019	0.288
Total number of observations	195094
Number of reflections	29246
<I/σ(I)>	9.8
Completeness [%]	98.3	97.6	94.6
Redundancy	6.7	5.9	6.6
CC(1/2)		0.998	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1M Lithium Sulfate monohydrate, 0.1M HEPES-Sodium, 0.1M Potassium Sodium Tartrate