5CA6
Crystallographic structure of apo porcine rotavirus TFR-41 VP8*
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.954 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.816, 30.444, 86.217 |
| Unit cell angles | 90.00, 124.05, 90.00 |
Refinement procedure
| Resolution | 35.800 - 1.900 |
| R-factor | 0.16616 |
| Rwork | 0.164 |
| R-free | 0.20254 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i2s |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.228 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.910 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.148 | |
| Number of reflections | 23031 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 98.4 | 89.5 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.4 and 2 M ammonium formate |
