5C8A
Crystal structure of a truncated form of Thermus thermophilus CarH bound to adenosylcobalamin (dark state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.430, 99.680, 143.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.958 - 2.150 |
| R-factor | 0.1853 |
| Rwork | 0.183 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SAD solution from different space group |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.819 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 40511 | |
| <I/σ(I)> | 15.2 | 2.1 |
| Completeness [%] | 98.5 | 99.4 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% PEG 3350, 0.2 M KCl |
