5C7N
Crystal structure of the human BRPF1 bromodomain in complex with Bromosporine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-20 |
Detector | PSI PILATUS 2M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.502, 60.502, 63.111 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.310 - 1.750 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.22060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lc2 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.987 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.310 |
High resolution limit [Å] | 1.750 |
Rmerge | 0.044 |
Number of reflections | 13890 |
<I/σ(I)> | 27.5 |
Completeness [%] | 100.0 |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 20% PEG3350, 0.1 M sodium acetate, 0.15 M sodium nitrate, pH 5.5 |