5C79
PH domain of ASAP1 in complex with diC4-PtdIns(4,5)P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-07-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.543, 64.697, 44.428 |
| Unit cell angles | 90.00, 95.63, 90.00 |
Refinement procedure
| Resolution | 44.210 - 1.600 |
| R-factor | 0.20018 |
| Rwork | 0.198 |
| R-free | 0.23345 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.889 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 27403 |
| <I/σ(I)> | 15.3 |
| Completeness [%] | 97.6 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1M HEPES, pH 7.5, 15% PEG 3350 |
