5C73
ATP-driven lipid-linked oligosaccharide flippase PglK in outward-occluded conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2014-12-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 200.030, 200.030, 693.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.938 - 5.900 |
R-factor | 0.286 |
Rwork | 0.284 |
R-free | 0.31710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5c78 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.861 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 5.800 | |
High resolution limit [Å] | 5.900 | 24.610 | 5.650 |
Rmerge | 0.197 | 0.047 | 2.200 |
Rmeas | 0.201 | 0.049 | 2.245 |
Total number of observations | 1600808 | ||
Number of reflections | 32005 | 424 | 646 |
<I/σ(I)> | 12.97 | 50.22 | 1.74 |
Completeness [%] | 84.6 | 43.4 | 10.3 |
Redundancy | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | Na/K phosphate; NaCl; Ammonium sulfate; ADP; MgCl2 |