5C65
Structure of the human glucose transporter GLUT3 / SLC2A3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.550, 116.030, 99.750 |
| Unit cell angles | 90.00, 106.91, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.650 |
| R-factor | 0.2062 |
| Rwork | 0.205 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pyp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.020 |
| Data reduction software | xia2 (0.3.6.3) |
| Data scaling software | Aimless (0.5.9) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.710 | 2.720 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.088 | 1.139 |
| Number of reflections | 40188 | |
| <I/σ(I)> | 16.8 | 1.4 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 7.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M citrate pH 5.5, 29% PEG400, 0.1M lithium sulfate |
