5C5B
Crystal Structure of Human APPL BAR-PH Heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-28 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.924, 88.542, 147.388 |
| Unit cell angles | 90.00, 92.65, 90.00 |
Refinement procedure
| Resolution | 49.077 - 2.900 |
| R-factor | 0.2135 |
| Rwork | 0.210 |
| R-free | 0.26820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.971 |
| Data reduction software | HKL-2000 (0.95) |
| Data scaling software | HKL-2000 (0.95) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.800 | |
| Rmerge | 0.112 | |
| Number of reflections | 46308 | |
| <I/σ(I)> | 14.89 | 1.72 |
| Completeness [%] | 98.4 | 90.3 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.2 M disodium hydrogen phosphate, 18% (w/v) PEG3350, 100 mM HEPES, and 1 mM DTT |
