5C3U
Crystal structure of a fungal L-serine ammonia-lyase from Rhizomucor miehei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.980, 72.885, 111.121 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.560 - 1.760 |
| R-factor | 0.18756 |
| Rwork | 0.186 |
| R-free | 0.20942 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p5j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.363 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.560 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.079 | |
| Number of reflections | 29066 | |
| <I/σ(I)> | 45.31 | 3.49 |
| Completeness [%] | 97.5 | 91.48 |
| Redundancy | 6.5 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.2 M KCl, 35% 5/4, PO/OH, and 50 mM HEPES pH 7.5 |
