5C3U

Crystal structure of a fungal L-serine ammonia-lyase from Rhizomucor miehei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-20
Detector	MAR CCD 165 mm
Wavelength(s)	0.9791
Spacegroup name	C 2 2 21
Unit cell lengths	72.980, 72.885, 111.121
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.560 - 1.760
R-factor	0.18756
Rwork	0.186
R-free	0.20942
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p5j
RMSD bond length	0.009
RMSD bond angle	1.363
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.560	1.820
High resolution limit [Å]	1.760	1.760
Rmerge	0.079
Number of reflections	29066
<I/σ(I)>	45.31	3.49
Completeness [%]	97.5	91.48
Redundancy	6.5	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	0.2 M KCl, 35% 5/4, PO/OH, and 50 mM HEPES pH 7.5