5C36
Crystal structure of GTA + UDP-C-Gal + DI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2007-10-25 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.550, 149.070, 79.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.550 |
R-factor | 0.1781 |
Rwork | 0.176 |
R-free | 0.21160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lz0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.516 |
Data reduction software | CrystalClear (1.3.6 SP2 r1) |
Data scaling software | d*TREK (9.4SSI) |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.910 | 19.910 | 1.610 |
High resolution limit [Å] | 1.550 | 3.330 | 1.550 |
Rmerge | 0.042 | 0.021 | 0.287 |
Total number of observations | 206174 | 22743 | 16892 |
Number of reflections | 45201 | ||
<I/σ(I)> | 16.2 | 48.9 | 3.9 |
Completeness [%] | 98.8 | 94 | 100 |
Redundancy | 4.53 | 5 | 3.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | Grown in 1% polyethylene glycol (PEG) 4000, 4.5-5% 2-methyl-2,4-pentanediol (MPD), 100 mM ammonium sulfate, 70 mM sodium chloride, 50 mM N-[-acetamido]-2-iminodiacetic acid (ADA), 30 mM sodium acetate, 5 mM manganese chloride. Grown 5-10 days. |