5C2B
anti-CXCL13 parental scFv - 3B4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2013-08-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 131.900, 133.500, 44.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.975 - 1.405 |
R-factor | 0.1798 |
Rwork | 0.179 |
R-free | 0.19440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | E10 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.067 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.480 |
High resolution limit [Å] | 1.405 | 1.405 |
Rmerge | 0.037 | 0.542 |
Number of reflections | 77347 | |
<I/σ(I)> | 22.5 | 3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.5 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | The concentration of 3B4 was 11 mg/ml in 20 mM Tris, pH 7.5 and 150 mM NaCl. Each drop contained 0.15 ul 3B4 and 0.15 ul reservoir solution containing 100 mM HEPES, pH 7.5 and 4300 mM NaCl. |