5C27
Crystal structure of SYK in complex with compound 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.879, 84.875, 89.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.430 - 2.150 |
| R-factor | 0.1795 |
| Rwork | 0.177 |
| R-free | 0.22510 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 90.000 |
| High resolution limit [Å] | 2.150 |
| Number of reflections | 17174 |
| <I/σ(I)> | 19 |
| Completeness [%] | 99.8 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 298 | 1.75 M ammonium sulfate, 0.1 M sodium citrate, 0.1 M sodium acetate pH 5.5 |
