5C1V
CRYSTAL STRUCTURE ANALYSIS OF CATALYTIC SUBUNIT OF HUMAN CALCINEURIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-02 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 185.008, 185.008, 106.744 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.800 - 3.350 |
R-factor | 0.217 |
Rwork | 0.216 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aui |
RMSD bond length | 0.017 |
RMSD bond angle | 2.122 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.800 | 3.530 |
High resolution limit [Å] | 3.350 | 3.350 |
Rmerge | 0.184 | 0.884 |
Number of reflections | 15994 | |
<I/σ(I)> | 13 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 17.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1 M HEPES, 25% PEG 3350, PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1 M HEPES, 25% PEG 3350, PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |