5C1D
Human OGT in complex with UDP-5S-GlcNAc and substrate peptide (RB2L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 138.527, 151.611, 200.429 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.200 - 2.050 |
| R-factor | 0.19625 |
| Rwork | 0.195 |
| R-free | 0.22864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pe4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.381 |
| Data reduction software | XDS (0.5) |
| Data scaling software | SCALA (3.3.21) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.070 | 0.826 |
| Number of reflections | 65809 | |
| <I/σ(I)> | 17.4 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 294.15 | 1.45 M Potassium diohydrogen phosphate, 8 mM EDTA, 1% xylitol (reservoir). 1.45 M Potassium diohydrogen phosphate, 8 mM EDTA, 1% xylitol, 0.5 M Ammonium sulfate (drop) |
