5C16

Myotubularin-related proetin 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 1
Unit cell lengths	67.219, 96.587, 97.581
Unit cell angles	87.60, 86.07, 77.33

Refinement procedure

Resolution	34.814 - 2.070
R-factor	0.2229
Rwork	0.220
R-free	0.27350
RMSD bond length	0.008
RMSD bond angle	1.040
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.000	2.070
Rmerge		0.255
Number of reflections	136495
<I/σ(I)>	18.9	3.2
Completeness [%]	86.1	84
Redundancy	2.5	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Sodium-HEPES, PEG 20000