5C0N
Development of a monoclonal antibody targeting secreted aP2 to treat diabetes and fatty liver disease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2013-05-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.270, 101.950, 95.320 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 54.970 - 3.000 |
| R-factor | 0.19425 |
| Rwork | 0.186 |
| R-free | 0.26464 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lie |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.622 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.970 | 3.090 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.180 | |
| Number of reflections | 26312 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | 0.1M Hepes pH 7.5, 0.2M NH2SO4, 16% PEG 4K and 10% isopropanol |
