5C02
Influenza A M2 transmembrane domain drug-resistant S31N mutant at pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 4 |
| Unit cell lengths | 28.650, 28.650, 68.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.837 - 1.591 |
| R-factor | 0.1631 |
| Rwork | 0.160 |
| R-free | 0.19720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c9j |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.712 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.190 | 1.640 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.041 | 0.243 |
| Number of reflections | 3670 | |
| <I/σ(I)> | 17.2 | 3.3 |
| Completeness [%] | 98.9 | 94.3 |
| Redundancy | 3.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 283 | calcium chloride, Tris HCl pH 8.0, PEG 400, strontium chloride |
