5BZT
Crystal structure of the murine cd44 hyaluronan binding domain complex with a small molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.791, 81.779, 32.133 |
| Unit cell angles | 90.00, 117.98, 90.00 |
Refinement procedure
| Resolution | 5.797 - 1.250 |
| R-factor | 0.1791 |
| Rwork | 0.178 |
| R-free | 0.19240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.341 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 5.797 | 1.254 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.102 | |
| Number of reflections | 36555 | |
| <I/σ(I)> | 26.6 | 11.6 |
| Completeness [%] | 94.3 | 91 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG MME 5000, MES, (NH4)2SO4 |
