5BZ4
Crystal structure of a T1-like thiolase (CoA-complex) from Mycobacterium smegmatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-08-06 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5 |
Spacegroup name | P 61 |
Unit cell lengths | 190.076, 190.076, 265.084 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.519 - 2.430 |
R-factor | 0.1641 |
Rwork | 0.161 |
R-free | 0.18750 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.242 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.520 |
High resolution limit [Å] | 2.430 |
Number of reflections | 201832 |
<I/σ(I)> | 8.2 |
Completeness [%] | 98.9 |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6 | 293 | 45% PEG 200, 0.1M MES monohydrate pH 6.0, 0.07M calcium chloride dehydrate, 5mM CoA |