5BYG

X-ray structure of AAV2 OBD-AAVS1 complex 2:1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	110
Detector technology	CCD
Collection date	2013-03-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	183.136, 79.595, 139.502
Unit cell angles	90.00, 98.12, 90.00

Refinement procedure

Resolution	29.396 - 2.500
R-factor	0.1914
Rwork	0.189
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zo0
RMSD bond length	0.009
RMSD bond angle	1.114
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge		0.698
Number of reflections	65724
<I/σ(I)>	18.7	1.5
Completeness [%]	99.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	Per-6K, Sodium Citrate, Lithium Chloride