5BY9
The crystal structure of polyglycilated 14-3-3 protein from Giardia intestinalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 32 |
| Unit cell lengths | 100.207, 100.207, 140.955 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.950 - 4.000 |
| R-factor | 0.2326 |
| Rwork | 0.233 |
| R-free | 0.23340 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.044 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 8.590 | 4.000 |
| Rmerge | 0.154 | 0.035 | 0.406 |
| Total number of observations | 23764 | ||
| Number of reflections | 12386 | ||
| <I/σ(I)> | 5.3 | ||
| Completeness [%] | 92.5 | 90.4 | 84.4 |
| Redundancy | 1.9 | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | LiCl 1M, hepes pH 7, PEG6K 10% |
