5BWY

Structure of proplasmepsin II from Plasmodium falciparum, Space Group P43212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-24
Detector	ADSC QUANTUM 1
Wavelength(s)	0.972
Spacegroup name	P 43 21 2
Unit cell lengths	89.380, 89.380, 115.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.690 - 2.644
R-factor	0.2059
Rwork	0.204
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pfz
RMSD bond length	0.003
RMSD bond angle	0.764
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	2.760
High resolution limit [Å]	2.640	2.640
Rmerge	0.080	0.700
Number of reflections	14252
<I/σ(I)>	9.9	1.6
Completeness [%]	100.0	100
Redundancy	2	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1 M Sodium Citrate, 0.1 M Sodium Acetate, 25% PEG 3350