5BWM
The complex structure of C3cer exoenzyme and GDP bound RhoA (NADH-bound state)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-19 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00 |
Spacegroup name | P 32 |
Unit cell lengths | 50.479, 50.479, 136.671 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.560 - 2.500 |
R-factor | 0.21103 |
Rwork | 0.209 |
R-free | 0.24596 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1A2B and 3BW8 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.737 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9) |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 13477 | |
<I/σ(I)> | 17.4 | 1.81 |
Completeness [%] | 99.6 | 100 |
Redundancy | 4.7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM MES (pH 6.4), 20% PEG1500 |