5BVI
X-ray Structure of Interferon Regulatory Factor 4 IAD Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 193.15 |
Detector technology | PIXEL |
Collection date | 2013-10-18 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 45.533, 84.854, 149.851 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.337 - 2.600 |
R-factor | 0.1991 |
Rwork | 0.194 |
R-free | 0.25550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dsh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.145 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.400 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.153 | 0.660 |
Number of reflections | 23107 | |
<I/σ(I)> | 8.7 | 2.1 |
Completeness [%] | 93.4 | 89.1 |
Redundancy | 4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 1.5-1.7M KCl, 0.1M Imidazole pH 8.0 |