5BV5
Structure of CYP119 with T213A and C317H mutations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.486, 137.871, 91.779 |
| Unit cell angles | 90.00, 101.18, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.24038 |
| Rwork | 0.238 |
| R-free | 0.27673 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.852 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 40139 | |
| <I/σ(I)> | 10.7 | 1.6 |
| Completeness [%] | 98.5 | 97.6 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 298 | 0.1 M Tris-HCl, 5 % PEG 4000 |
