5BTF
Crystal structure of a topoisomerase II complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.955, 84.019, 130.590 |
| Unit cell angles | 90.00, 108.87, 90.00 |
Refinement procedure
| Resolution | 49.776 - 2.610 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bs8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.699 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.780 | 2.703 | |
| High resolution limit [Å] | 2.610 | 11.670 | 2.610 |
| Rmerge | 0.145 | 0.035 | |
| Rmeas | 0.175 | 0.049 | 1.714 |
| Total number of observations | 224013 | ||
| Number of reflections | 224023 | 1332 | 8615 |
| <I/σ(I)> | 6.65 | 15.17 | 0.86 |
| Completeness [%] | 97.2 | 88.9 | 93.62 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 100 mM Tris-HCl, 13-15% PEG 4000, 200 mM MgCl2, 6.25% PEG 400 |
