5BS8

Crystal structure of a topoisomerase II complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	108.357, 83.070, 129.856
Unit cell angles	90.00, 109.33, 90.00

Refinement procedure

Resolution	102.252 - 2.399
R-factor	0.1976
Rwork	0.196
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xkk
RMSD bond length	0.004
RMSD bond angle	0.686
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.300	2.485
High resolution limit [Å]	2.399	2.399
Rmerge	0.088
Number of reflections	83492
<I/σ(I)>	10.66	0.99
Completeness [%]	97.7	93.22
Redundancy	3.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	100 mM Tris-HCl, 13-15% PEG 4000, 200 mM MgCl2, 6.25% PEG 400