5BQD
Crystal Structure of TBX5 (1-239) Dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2011-11-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.977 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 81.455, 81.455, 341.022 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.033 - 2.583 |
R-factor | 0.2503 |
Rwork | 0.246 |
R-free | 0.28890 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.259 |
Data reduction software | HKL-2000 (704k) |
Data scaling software | HKL-2000 (704k) |
Phasing software | PHENIX (1.9-1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.230 |
High resolution limit [Å] | 2.583 |
Number of reflections | 21874 |
<I/σ(I)> | 8.88 |
Completeness [%] | 92.1 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 298 | 100mM Sodium Cacodylate pH 6.4 10mM MgSO4 1.8M Ammonium Sulphate |