5BOZ

Ricin A chain bound to camelid nanobody (VHH9)(E1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	F 2 2 2
Unit cell lengths	239.610, 242.884, 355.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.681 - 3.100
R-factor	0.2177
Rwork	0.215
R-free	0.27710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.818
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.681	50.000	3.100
High resolution limit [Å]	2.930	7.940	2.980
Rmerge	0.200	0.072
Rmeas	0.219	0.079
Rpim	0.118	0.032	0.973
Total number of observations	640340
Number of reflections	109638
<I/σ(I)>	2.4
Completeness [%]	99.8	98.9	99.9
Redundancy	5.8	5.6	5.6
CC(1/2)		0.996	0.562

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	1.45 M Ammonium Sulfate, 25% glycerol