5BOZ
Ricin A chain bound to camelid nanobody (VHH9)(E1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 239.610, 242.884, 355.693 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.681 - 3.100 |
R-factor | 0.2177 |
Rwork | 0.215 |
R-free | 0.27710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.818 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.681 | 50.000 | 3.100 |
High resolution limit [Å] | 2.930 | 7.940 | 2.980 |
Rmerge | 0.200 | 0.072 | |
Rmeas | 0.219 | 0.079 | |
Rpim | 0.118 | 0.032 | 0.973 |
Total number of observations | 640340 | ||
Number of reflections | 109638 | ||
<I/σ(I)> | 2.4 | ||
Completeness [%] | 99.8 | 98.9 | 99.9 |
Redundancy | 5.8 | 5.6 | 5.6 |
CC(1/2) | 0.996 | 0.562 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 1.45 M Ammonium Sulfate, 25% glycerol |