5BOW
CRYSTAL STRUCTURE OF IL-38
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 31.264, 62.514, 62.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.270 - 1.310 |
| R-factor | 0.139 |
| Rwork | 0.138 |
| R-free | 0.16000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.145 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.330 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmerge | 0.098 | 0.403 |
| Number of reflections | 29448 | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 97.4 | 89.1 |
| Redundancy | 11.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | PEG 3350, NAACETATE, PH 5.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K |
