5BOL
DNA polymerase beta ternary complex with a templating 5ClC and incoming dGTP analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-01 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.640, 79.114, 55.702 |
| Unit cell angles | 90.00, 107.65, 90.00 |
Refinement procedure
| Resolution | 25.520 - 1.981 |
| R-factor | 0.1925 |
| Rwork | 0.190 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.828 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.064 | 0.503 |
| Total number of observations | 102131 | |
| Number of reflections | 28993 | |
| <I/σ(I)> | 13 | 2.16 |
| Completeness [%] | 99.3 | 95.9 |
| Redundancy | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 50mM Imidazole, 350mM Sodium Acetate, 18% Peg3350, pH 8.0, VAPOR DIFFUSION, temperature 291K |
