5BMU
The crystal structure of the GST-like domains complex of AIMP3-EPRS mutant C92SC105SC123S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | P 31 |
Unit cell lengths | 92.063, 92.063, 185.948 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.930 - 2.600 |
R-factor | 0.13917 |
Rwork | 0.138 |
R-free | 0.16957 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bvx |
RMSD bond length | 0.011 |
RMSD bond angle | 1.538 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 53953 | |
<I/σ(I)> | 16.5 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.4 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293.15 | Ammonium sulfate, Sodium chloride, Tris-HCl |