5BK3
Crystal structure of the neutralizing anti-circumsporozoite protein 580 antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-16 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.364, 179.567, 140.430 |
| Unit cell angles | 90.00, 109.61, 90.00 |
Refinement procedure
| Resolution | 39.578 - 2.800 |
| R-factor | 0.1914 |
| Rwork | 0.190 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i18 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.945 |
| Data reduction software | XDS |
| Data scaling software | SHELXPREP |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rpim | 0.074 | 0.345 |
| Number of reflections | 62085 | 6197 |
| <I/σ(I)> | 9.95 | 1.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 294 | 100 mM MES pH 5, 1.6 M (NH4)2SO4 |
