5BJO

Crystal structure of the Corn RNA aptamer in complex with DFHO, site-specific 5-iodo-U

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.5498
Spacegroup name	P 21 21 21
Unit cell lengths	36.269, 55.305, 99.002
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.500 - 2.350
R-factor	0.18448
Rwork	0.180
R-free	0.22822
RMSD bond length	0.009
RMSD bond angle	1.905
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Number of reflections	7797
<I/σ(I)>	32.1
Completeness [%]	99.1
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	30% PEG 550 MME, 0.1 M Tris-HCl pH 8.05