5B8B
Crystal structure of reduced chimeric E.coli AhpC1-186-YFSKHN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.999999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 100.494, 135.654, 106.489 |
Unit cell angles | 90.00, 111.22, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.100 |
R-factor | 0.25033 |
Rwork | 0.249 |
R-free | 0.28366 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n8j |
RMSD bond length | 0.007 |
RMSD bond angle | 0.946 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 44808 | |
<I/σ(I)> | 8.9 | |
Completeness [%] | 92.9 | 92 |
Redundancy | 3.1 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.6 M ammonium sulfate, 100 mM MES pH 6.5, 5% Dioxane |