5B83
Crystal structure of Optineurin UBAN in complex with linear ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-07 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.327, 82.038, 244.853 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.061 - 2.694 |
R-factor | 0.2039 |
Rwork | 0.201 |
R-free | 0.25410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zvn |
RMSD bond length | 0.010 |
RMSD bond angle | 1.131 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.860 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.141 | 0.920 |
Number of reflections | 40450 | |
<I/σ(I)> | 12.6 | 2.2 |
Completeness [%] | 99.4 | 96.3 |
Redundancy | 7.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 16% PEG 3350, 250mM Potassium formate |