5B5K
Crystal structure of Izumo1, the mammalian sperm ligand for egg Juno
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98400 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 103.280, 103.280, 139.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.840 - 2.500 |
| R-factor | 0.2577 |
| Rwork | 0.257 |
| R-free | 0.28070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jk9 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.809 |
| Data reduction software | XDS (20151015) |
| Data scaling software | XDS (20151015) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.840 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.195 | 0.230 |
| Number of reflections | 13389 | |
| <I/σ(I)> | 12.13 | 1.13 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.6 | 10.1 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 293 | 0.2 M Ammonium formate pH 6.6, 20% PEG 3350 |
