5B4B
Crystal structure of LpxH with lipid X in spacegroup C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.534, 42.484, 100.756 |
| Unit cell angles | 90.00, 125.12, 90.00 |
Refinement procedure
| Resolution | 49.780 - 1.600 |
| R-factor | 0.16241 |
| Rwork | 0.161 |
| R-free | 0.19008 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b49 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.451 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 65538 | |
| <I/σ(I)> | 18.3 | 1.97 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 131mM DL-Malic acid pH7.0, 17.5% PEG 3350, 10% (v/v) glycerol |
