5B4A
Crystal structure of LpxH with lipid X in spacegroup P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.016, 108.805, 51.685 |
| Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
| Resolution | 25.640 - 1.720 |
| R-factor | 0.1611 |
| Rwork | 0.160 |
| R-free | 0.18824 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b49 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.375 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 57104 | |
| <I/σ(I)> | 18.2 | 2.01 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.2M L-Proline, 0.1M HEPES pH 7.4, 9.0% PEG 3350, 5% glycerol |
