5B3J
Activation of NMDA receptors and the mechanism of inhibition by ifenprodil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-04-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 247.249, 79.903, 181.314 |
| Unit cell angles | 90.00, 127.09, 90.00 |
Refinement procedure
| Resolution | 29.930 - 2.900 |
| R-factor | 0.275 |
| Rwork | 0.273 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.068 |
| Data reduction software | HKL-2000 |
| Data scaling software | PHENIX |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.099 |
| Number of reflections | 57592 |
| <I/σ(I)> | 8.5 |
| Completeness [%] | 91.4 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 0.1M sodium acetate, 27% PEG3350, 2.2M sodium formate, 0.05 M calcium chloride |
