5B2Z
H-Ras WT in complex with GppNHp (state 2*) before structural transition by humidity control
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 87.974, 87.974, 132.966 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.400 - 1.560 |
| R-factor | 0.14974 |
| Rwork | 0.148 |
| R-free | 0.17784 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k8y |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.510 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.590 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.062 | 0.521 |
| Number of reflections | 28446 | |
| <I/σ(I)> | 47.2 | 6.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | PEG400, SODIUM ACETATE, CALCIUM ACETATE, MAGNESIUM CHLORIDE |
