5B2K
A crucial role of Cys218 in the stabilization of an unprecedented auto-inhibition form of MAP2K7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-30 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 71.394, 71.394, 261.996 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.960 - 2.750 |
R-factor | 0.27261 |
Rwork | 0.270 |
R-free | 0.32761 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wzu |
RMSD bond length | 0.012 |
RMSD bond angle | 1.766 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.750 |
Number of reflections | 11135 |
<I/σ(I)> | 22.7 |
Completeness [%] | 95.0 |
Redundancy | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | PEG 3350, sodium citrate tribasic, Hepes |