5B2K
A crucial role of Cys218 in the stabilization of an unprecedented auto-inhibition form of MAP2K7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-30 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 71.394, 71.394, 261.996 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.960 - 2.750 |
| R-factor | 0.27261 |
| Rwork | 0.270 |
| R-free | 0.32761 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wzu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.766 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 11135 |
| <I/σ(I)> | 22.7 |
| Completeness [%] | 95.0 |
| Redundancy | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | PEG 3350, sodium citrate tribasic, Hepes |
