5B1H
Crystal structure of cystathionine beta-synthase from Lactobacillus plantarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 139.890, 146.340, 82.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.490 - 2.400 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2q3d |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | AMoRE |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.480 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.064 | 0.412 |
Number of reflections | 62935 | |
<I/σ(I)> | 19.8 | 2 |
Completeness [%] | 97.6 | 85 |
Redundancy | 3.4 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | lithium sulfate, hepes |