5B06

Lysozyme (denatured by NaOD and refolded)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-05-08
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	78.800, 78.800, 36.801
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.720 - 1.800
R-factor	0.15088
Rwork	0.149
R-free	0.18745
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yvb
RMSD bond length	0.021
RMSD bond angle	1.796
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.800
Number of reflections	11257
<I/σ(I)>	80.7
Completeness [%]	100.0
Redundancy	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	NaCl, ethylene glycol