5AZG
Crystal structure of LGG-1 complexed with a UNC-51 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-09-28 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.684, 80.116, 111.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.750 - 1.810 |
| R-factor | 0.21339 |
| Rwork | 0.212 |
| R-free | 0.24608 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5azf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.800 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.878 | |
| Number of reflections | 26489 | |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | sodium acetate, cadmium chloride |
