5AZG

Crystal structure of LGG-1 complexed with a UNC-51 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	95
Detector technology	CCD
Collection date	2015-09-28
Detector	RAYONIX MX-225
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	63.684, 80.116, 111.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.750 - 1.810
R-factor	0.21339
Rwork	0.212
R-free	0.24608
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5azf
RMSD bond length	0.008
RMSD bond angle	1.190
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.800	1.830
High resolution limit [Å]	1.800	1.800
Rmerge		0.878
Number of reflections	26489
<I/σ(I)>	18.1
Completeness [%]	99.9	100
Redundancy	6.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	sodium acetate, cadmium chloride