5AZC

Crystal structure of Escherichia coli Lgt in complex with phosphatidylglycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	50.117, 60.885, 117.448
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.520 - 1.902
R-factor	0.2137
Rwork	0.212
R-free	0.25070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.108
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.900
Number of reflections	28535
<I/σ(I)>	19.3
Completeness [%]	98.3
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Tris, CaCl2, PEG2000MME