5AZB
Crystal structure of Escherichia coli Lgt in complex with phosphatidylglycerol and the inhibitor palmitic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9200 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.413, 60.973, 117.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.426 - 1.600 |
R-factor | 0.1813 |
Rwork | 0.179 |
R-free | 0.22180 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.035 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1634)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
Rmerge | 0.067 | 0.035 | |
Rmeas | 0.069 | 0.037 | |
Rpim | 0.020 | 0.010 | 0.359 |
Total number of observations | 607413 | ||
Number of reflections | 49244 | ||
<I/σ(I)> | 8 | ||
Completeness [%] | 99.9 | 99.9 | 99.1 |
Redundancy | 12.3 | 12.2 | 8.6 |
CC(1/2) | 0.999 | 0.478 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris , CaCl2, and PEG2000 MME |