5AZ2

Crystal structure of the Fab fragment of 9E5, a murine monoclonal antibody specific for human epiregulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-07
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	41.000, 79.830, 59.980
Unit cell angles	90.00, 92.59, 90.00

Refinement procedure

Resolution	17.274 - 1.603
R-factor	0.1949
Rwork	0.193
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.181
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	48127
<I/σ(I)>	16.2
Completeness [%]	95.3
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	HEPES Na, PEG4000