Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.178, 137.122, 92.650 |
| Unit cell angles | 90.00, 103.76, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.800 |
| Rwork | 0.201 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jbm |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (6.5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 42775 | |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 96.7 | 94.2 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 294 | 100MM MES-NAOH (PH 5.0), 7-15% (W/V) PEG-MME 2000, 10MM KSCN, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 294K |
