5AYT
Crystal structure of transthyretin in complex with L6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-18 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 43.578, 84.814, 65.291 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.250 - 1.400 |
R-factor | 0.19047 |
Rwork | 0.189 |
R-free | 0.20890 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.667 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.250 |
High resolution limit [Å] | 1.400 |
Number of reflections | 46782 |
<I/σ(I)> | 27.4 |
Completeness [%] | 96.2 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 18% polyethylene glycol 400, 0.1 M HEPES-NaOH, 0.2 M CaCl2, 1 mM L6 |